Lee MY, Kim YK, Ryoo KK, Lee YB, Park EJ.
Department of Genetic Engineering, Soonchunhyang University, Asan, Chungnam 336-600, Korea. [email protected]
The generation of reactive oxygen species is thought to cause extensive oxidative damage to various bio molecules such as DNA, RNA, and protein. In this study, the preventive, suppressive, and protective effects of in vitro supplementation with electrolyzed-reduced water on H2O2-induced DNA damage in human lymphocytes were examined using a comet assay. Pretreatment, co-treatment, and post treatment with electrolyzed-reduced water enhanced human lymphocyte resistance to the DNA strand breaks induced by H2O2 in vitro. Moreover, electrolyzed-reduced water was much more effective than diethylpyrocarbonate-treated water in preventing total RNA degradation at 4 and 25 degrees C. In addition, electrolyzed-reduced water completely prevented the oxidative cleavage of horseradish peroxidase, as determined using sodium dodecyl sulfate-polyacrylamide gels. Enhancement of the antioxidant activity of ascorbic acid dissolved in electrolyzed-reduced water was about threefold that of ascorbic acid dissolved in non electrolyzed deionized water, as measured by a xanthine-xanthine oxidize superoxide scavenging assay system, suggesting an inhibitory effect of electrolyzed reduced water on the oxidation of ascorbic acid.
PMID: 17159237 [PubMed - indexed for MEDLINE]
